Laboratory Services

EGFR Mutation Analysis

Name: Incyte Diagnostics
Address: 13103 E Mansfield Ave, Spokane Valley, Washington, 99216, USA
Telephone: +1-

Test Description	EGFR Mutation Analysis
Synonym(s)	EGFR, epidermal growth factor receptor
Test ID	EGFRPCR
General Information	Bi-directional sequencing of exons 18-21 of the EGFR gene for detection of EGFR-activating mutations and TKI resistance mutations (including T790M) in these exons. Tumor enrichment is performed before extraction. EGFR mutation analysis is recommended in non-small cell lung carcinoma (NSCLC) to detect mutations (commonly L858R and exon 19 deletions) associated with increased sensitivity to EGFR tyrosine kinase inhibitors. Detection of TKI resistance mutations such as T790M in patients being treated with a TKI is useful for planning alternate treatment.
Specimen Type	FFPE solid tumor tissue: Paraffin block is preferred. Alternatively, send 1 H&E slide plus 5-10 unstained slides cut at 5 or more microns. Please use positively-charged slides and 10% NBF fixative. Do not use zinc fixatives.
Required Information	Complete the Incyte Diagnostics requisition with the patient's demographics, ordering physician, and a copy of the pathology report.
Stability	Use cold pack for transporting block during summer to prevent block from melting. Slides can be packed at room temperature
Methodology	Molecular Pathology
Estimated TAT	8 Days
Retention	Extracted DNA is kept for 30 days
CPT Code(s)	81235
Performing Lab	NeoGenomics Laboratories