Lactic Acid

Lactic Acid

Anaerobic glycolysis markedly increases blood lactate, especially with prolonged exercise. The common cause for increased blood lactate is anoxia resulting from such conditions as shock, pneumonia and congestive heart failure. Lactic acidosis may also occur in renal failure and leukemia. Thiamine deficiency and diabetic ketoacidosis are associated with increased levels of lactate and pyruvate. Lactate measurements that evaluate the acid‑base status are used in the diagnosis and treatment of lactic acidosis (abnormally high acidity in the blood).

Plasma

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Heparinized plasma is acceptable, but precautions must be taken to retard glycolysis by keeping the whole blood on ice and then separating the plasma from the cells within 15 minutes of collection.

Centrifuge and aliquot plasma to an aliquot tube within 15 minutes of collection. Avoid the use of a tourniquet, if possible

1 mL

500 uL

Centrifuge and aliquot plasma to an aliquot tube within 15 minutes of collection. Avoid the use of a tourniquet, if possible

Stability in plasma (separated):
8 hours at 15‑25 °C
14 days at 2‑8 °C

Stability in plasma (heparinized):
38 days at -20 °C

Do not use serum specimens.

The lactate level increases rapidly with physical exercise. The time required for return to normal lactate values depends on the physical fitness of the subject. 30 minutes at rest is usually sufficient for this purpose.
Blood samples should be drawn from a stasis‑free vein. However, minimal hemostasis (less than 30 seconds) will not affect lactate levels. Avoid the use of a tourniquet, if possible.
Glycolysis in blood samples can rapidly increase lactate levels. Cells contribute to the glycolysis and their quick removal is essential for accurate lactate analysis.

Colorimetric

0-2 days

Monday-Saturday

83605

4.5-19.8 mg/dL

Incyte Diagnostics

2524-7