Third Generation LH assay; Ultrasensitive LH assay; Lutropin
Test ID
LHPED
General Information
Useful for diagnosis of precocious puberty and delayed puberty in children
Specimen Type
Serum
Specimen Requirements
Preferred: Red Top
Acceptable: Serum Gel (SST)
Specimen Collection / Processing Instructions
Red-top tubes should be centrifuged and the serum transferred to an aliquot vial within 2 hours of collection
Serum gel tubes should be centrifuged within 2 hours of collection.
Minimum Sample Volume
0.13 mL
Stability
Refrigerated (preferred): 14 days
Frozen: 90 days
Unacceptable Specimen Conditions
Gross hemolysis
Gross lipemia
Gross icterus
Limitations
No clinically significant cross-reactivity has been demonstrated with follicle-stimulating hormone, human chorionic gonadotropin, free alpha subunit of pituitary glycoprotein hormones, or free beta subunit of luteinizing hormone. Cross-reactivity with thyroid-stimulating hormone (TSH) (<5%) might be observed at TSH concentrations of 500 mIU/L
Some patients who have been exposed to animal antigens, either in the environment or as part of treatment or imaging procedures, may have circulating anti-animal antibodies present. These antibodies may interfere with the assay reagents to produce unreliable results
Methodology
Chemiluminescent Immunoassay (CIA)
Estimated TAT
2-9 days
Testing Schedule
Friday
Retention
3 months
CPT Code(s)
83002
Reference Range
Age (years)
Males (IU/L)
Females (IU/L)
< 1
<0.2 - 5.0
<0.2 - 18.3
1 - 8
<0.2 - 5.0
<0.2 - 0.3
9 - 10
<0.2 - 3.6
<0.2 - 4.8
11 - 13
0.1 - 5.7
<0.2 - 11.7
14 - 17
0.8 - 8.7
<0.2 - 16.7
Tanner Stage*
Stage I (1-8 years)
<0.2 - 5.0
<0.2 - 0.3
Stage II
0.03 - 3.7
<0.2 - 4.1
Stage III
0.09 - 4.2
0.6 - 7.2
Stage IV-V
1.3 - 9.8
0.9 - 13.3
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for girls at a median age of 10.5 (+/- 2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African-American girls. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (+/- 2) years. For boys there is no proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (adult) should be reached by age 18.
