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Semen Analysis, Semen

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Updated Test Information:

Test Description
Semen Analysis, Semen
Synonym(s)

Fertility Test, Semen Count, Semen Motility, Semen Volume, Sperm Analysis, Sperm Count, Sperm Motility, Sperm Volume
Test ID
FER
General Information

This test is for determining male fertility status.
Container Type

Semen Analysis Kit - Dilution Media (T178)
Specimen Type

Semen
Specimen Requirements

Patient Preparation: Patient should have 2 to 7 days of sexual abstinence at the time of semen collection for accurate results.
Specimen Volume: Total ejaculate


Patients must obtain a collection kit with collection instructions from one of the following Incyte Diagnostics Patient Service Centers

318 E Rowan Ave, Suite 205, Spokane, WA
9631 N Nevada St, Suite 210, Spokane, WA
750 N Syringa, Suite 101, Post Falls, ID
55 W Tietan St, Walla Walla, WA
221 Wellsian Way, Richland, WA



***Failure to obtain the collection kit from an Incyte Diagnostics Patient Service Center could result in rejection of the sample*** 
Specimen Collection / Processing Instructions

  1. After collection, allow the specimen to liquefy for 1 hour.

  2. Measure the volume.If necessary, also measure appearance, pH, and viscosity.

  3. Place the specimen into media within 1 hour.
Minimum Sample Volume

Total ejaculate
Required Information

Include the following information:
-Semen volume (required)
-Viscosity
-pH
-Appearance (color)
-Number of days of sexual abstinence
Stability

Ambient (preferred)


Specimen must arrive within 24 hours of collection. Send specimen Monday through Thursday only and not the day before a holiday. If holiday falls on a Saturday, holiday will be observed on the preceding Friday. Sunday holidays are observed on the following Monday. Specimen should be collected and packaged as close to shipping time as possible. Laboratory does not perform testing on weekends
Limitations

This test should not be used to check patient's sterility following a vasectomy. For such cases, order POSV / Post Vasectomy Check, Semen.


Semen analysis specimens submitted to Mayo Clinic Laboratories are not acceptable for fructose testing due to the use of dilution media. For specimen requirements for fructose testing in azoospermia patients, see FROS2 / Fructose, Qualitative, Semen.


 Submit separate specimen to rule-out ejaculatory duct blockage. Positive result indicates no blockage.
Methodology

The sample is measured for volume and analyzed microscopically to determine the number of sperm present, the number of moving or motile sperm, and the properties of the sperm motility.(WHO laboratory manual for the examination of human semen. 6th ed. World Health Organization; 2021)
Estimated TAT

2 to 5 Days
Testing Schedule

Monday - Friday
Retention

Not retained.
CPT Code(s)

89310
Reference Range

Appearance: normal
Volume: > or =1.5 mL
pH: > or =7.2
Motile/mL: > or =6.0 x 10(6)
Sperm/mL: > or =15.0 x 10(6)
Motility: > or =40%
Grade: > or =2.5
Note: Multiple laboratory studies have indicated that semen parameters for motility and grade on average retain 80% of original parameters when our shipping method is used for transport. Using these averages, samples with 32% to 39% motility and grade of 2 may be in the normal range if testing was performed shortly after collection. Therefore, these borderline patients may need to collect another sample at a local fertility center to verify fertility status.
Motile/ejaculate: > or =9.0 x 10(6)
Viscosity: > or =3.0
Agglutination: > or =3.0
Supravital: > or =58% live
Fructose: positive
Note: Fructose testing cannot be performed on semen analysis specimens shipped through Mayo Clinic Laboratories.
Submit separate specimen to rule out ejaculatory duct blockage. Positive result indicates no blockage.
Performing Lab

Mayo Clinic
LOINC Code(s)

FER Semen Analysis             54231-6
ABSTN Abstinence               10587-4
CLST1 Collection Site         56816-2
TY Study Type                          54453-6
CNTN Container Type        74384-9
APP3 Appearance                 13359-5
VL53 Semen Volume           3160-9
PH1 pH                                        2752-4
MOTML Motile/mL              42531-4
SPML Sperm/mL                    9780-8
MOTY Motility                       6800-7
GR2 Grade                                13942-8
MOTEJ Motile/Ejaculate  6800-7
VISC Viscosity                        32789-0
AGGLU Agglutination       33217-1
STAIN Supravital Stain     4466-9
FRCT Fructose                      13943-6
CMT45 Comment              48767-8