Laboratory Services

Testosterone Free and Total

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Updated Test Information:

Test Description
Testosterone Free and Total
Synonym(s)

Free testosterone

Test ID
FREET
General Information

Albumin, SHBG, Testosterone, Free Testosterone (Calculated)

Specimen Type

Serum

Specimen Requirements

SST

Alternate Specimen Type

Red top, Li-heparin plasma

Specimen Collection / Processing Instructions

Separate serum or plasma from cells within 45 minutes of collection. If a red-top tube or green-top tube is used, transfer separated serum or plasma to a plastic transport tube.

Minimum Sample Volume

0.5 mL

Stability

5 days at 20‑25 °C
7 days at 2‑8 °C
4 months at -20 ˚C

Unacceptable Specimen Conditions

gross hemolysis; lipemia; improper labeling

Methodology

Electrochemiluminescence immunoassay

Estimated TAT

0-2 Days

Testing Schedule

M-F

CPT Code(s)

82040
84270
84403

Reference Range

Free: Males
18–69 years 35.0–155.0 pg/mL
70–89 years 30.0–135.0 pg/mL


Free For Pre-menopausal Females: 1.3-5.6 pg/mL


TESTOSTERONE, TOTAL:
Males 
0 -5 months: 75-400 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-130 ng/dL
12-13 years: <7-800 ng/dL
14 years: <7-1,200 ng/dL
15-16 years: 100-1,200 ng/dL
17-18 years: 300-1,200 ng/dL
> or =19 years: 240-950 ng/dL


Females
0-5 months: 20-80 ng/dL
6 months-9 years: <7-20 ng/dL
10-11 years: <7-44 ng/dL
12-16 years: <7-75 ng/dL
17-18 years: 20-75 ng/dL
> or =19 years: 8-60 ng/dL


 Tanner Stages*
Male
I (prepubertal): <7-20
II: 8-66
III: 26-800
IV: 85-1,200
V (young adult): 300-950

Female
I (prepubertal): <7-20
II: <7-47
III: 17-75
IV: 20-75
V (young adult): 12-60


*Puberty onset (transition from Tanner stage I to Tanner stage II) occurs for boys at a median age of 11.5 (+/-2) years and for girls at a median age of 10.5 (+/-2) years. There is evidence that it may occur up to 1 year earlier in obese girls and in African American girls. For boys, there is no definite proven relationship between puberty onset and body weight or ethnic origin. Progression through Tanner stages is variable. Tanner stage V (young adult) should be reached by age 18.

Performing Lab

Incyte Diagnostics

LOINC Code(s)

1751-7, 13967-5, 2986-8, 2991-8