Tissue Transglutaminase (tTG) Antibody, IgG

tTG, Celiac

Tissue transglutaminase (tTG) has been identified as the major autoantigen in celiac disease and dermatitis herpetiformis. tTG IgG antibodies are less disease specific but can be useful markers in patients with IgA deficiency. Assay results should be used in conjunction with clinical findings and other serological tests. Antibodies directed against gliadin, its deamidated products, and tTG are dependent on the ingestion of gluten. For this reason, serology testing is best performed in patients on a gluten-containing diet. In children younger than 2 years, it is recommended to combine tissue transglutaminase (tTG) testing with deamidated gliadin (DGP) IgA and IgG.

Serum

Tiger

SST

Allow serum tubes to clot for a minimum of 30 minutes before centrifuging. Centrifuge within 1 hour of collection and no longer than 2 hours after collection. Failure to separate red cells from serum with 2 hours of collection may lead to inaccurate results.

500 uL

Caution: Serum samples are not to be repeatedly frozen and thawed, since this can cause analyte deterioration. They are to be thawed only once.

Stored at 22˚C: 8 hrs
Stored at 2-8˚C: 2 weeks
If assays are not completed within 2 weeks or the specimen is to be stored beyond 2 weeks, the serum/plasma should be frozen at or below -20˚C.

Plasma, lipemic, hemolyzed or microbially contaminated samples.

In children younger than 2 years, it is recommended to combine tissue transglutaminase (tTG) testing with deamidated gliadin (DGP) IgA and IgG.

Antibodies directed against gliadin, its deamidated products, and tTG are dependent on the ingestion of gluten. For this reason, serology testing is best performed in patients on a gluten-containing diet.

Fluorescent enzyme immunoassay (FEIA)

2-5 days

Monday-Friday, alternating days

86364

Negative < 7 U/mL
Equivocal 7-10 U/mL
Positive > 10 U/mL

Incyte Diagnostics

56537-4