Varicella Zoster Rapid PCR, Swab or CSF

UOW3701

The DiaSorin Simplexa™ Varicella Zoster Virus (VZV) Direct FDA approved assay is a rapid real-time PCR that enables the direct amplification, detection of VZV from both cutaneous and mucocutaneous swab specimens and CSF specimens. A well conserved region of the VZV DNA polymerase gene is targeted to identify VZV in the specimen. Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

Swab: Acceptable specimen types are cutaneous and mucocutaneous swabs stored in UTM, Remel M4, Remel M4RT, Remel M5, or Remel M6 transport media. Follow the manufacturers’ package inserts for collection media and acceptable swab types. Do not use calcium alginate swabs, as they may contain substances that inhibit PCR testing.

CSF: 1 mL in sterile container

Swab or CSF

 1 mL

Swabs: Transport specimens on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.

CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Transport CSF on ice, store at 2 to 8°C for up to 7 days post collection. If there is a greater than 7 days delay before testing of the specimen, store specimen at -60 to -85°C. Do not freeze/thaw.

 0.1 mL

Calcium alginate swabs, they may contain substances that inhibit PCR testing.

Clinical Virology

Real-time Polymerase Chain Reaction

Monday- Friday 07:00- 21:00, Saturday and Sunday 09:00-16:00

VZVRSD         Varicella Zoster PCR, Specimen Desc
VZVRR            Varicella Zoster PCR, Result
VZVCT            Varicella Zoster PCR, CT Value
VZVRIN         Varicella Zoster PCR, Interpretation

87798

UW

49451-8