Laboratory Services

Vitamin B12 and Folate

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Updated Test Information:

Test Description
Vitamin B12 and Folate
Synonym(s)

Folic Acid and Vitamin B12

Test ID
B12FOL
Performing Lab

Incyte Diagnostics

General Information

Aids in detection of vitamin B12 and folate deficiency in individuals with macrocytic or unexplained anemia, or unexplained neurologic disease.

Container Type

SST

Specimen Type

Serum

Specimen Requirements

1 mL B12, 1mL Folate

Alternate Specimen Type

Red top

Specimen Collection / Processing Instructions

SST - Samples should not be taken from patients receiving therapy with high biotin doses (i.e.greater than 5 mg/day) until at least 8 hours following the last biotin administration. Centrifuge within 45 minutes of collection and transfer serum for FOLATE to an Amber Light Protected aliquot tube and serum for B12 to a plastic transport tube.

Minimum Sample Volume

0.50 mL B12, 0.50 mL Folate

Pediatric Min. Volume (if applicable)

500 uL

Stability

2 hours at 15‑25 °C
48 hours at 2‑8 °C
56 days at ‑20 °C (± 5 °C). Freeze only once.

Stability of serum obtained with separating tubes: 24 hours at 2-8 °C

Unacceptable Specimen Conditions

Hemolyzed samples

Methodology

Eelectrochemiluminescence Immunoassay “ECLIA”

Estimated TAT

0-2 Days

Testing Schedule

Monday - Saturday

Test Includes

Vitamin B12, Folate

CPT Code(s)

82607
82746

Reference Range

B12: 232-1245 pg/mL
Folate: 4.78-24.2 ng/mL

LOINC Code(s)

2132-9, 2284-8